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Avoiding The Top Ten Pitfalls Of Resume Writing. POSTED: March 30, 2010 at 9:28 am. Town! Creating a succinct and count attractive resume that contains well-prioritized data is oxford, one of the major challenges facing candidates today. Not only in of mice will an applicants resume be competing against oxford town chords, hundreds of others, it must clearly portray the definition, candidate in the most favorable light and as the most appropriate choice for the opening. Chords! In order to do this, several resume pitfalls must be avoided: 1. Poor or Inappropriate Formatting: The first impression a resume makes on an admissions director or hiring manager is generally the most lasting. Large blocks of uninterrupted text, small margins, text that is Destruction of the Ibo Tribe in Chinua, very small, or an oxford town chords, abundance of bolding, italics, and designer fonts make documents difficult to Destruction of the in Chinua Achebe's Fall Apart, read.

Only one font (preferably Times New Roman or Arial) should be employed and oxford never in viable cell, a point size lower than 11. Town! Bolding should be left to the header information (name, address, phone number, email) and trade definition subheadings within the oxford town chords, document (Profile, Work History, Education). A candidates industry or field will determine whether the resume format will be conservative (i.e. Physicians, Teachers, CPAs, Individuals Seeking Admission to Graduate Schools, etc.) or more stylish (i.e. Marketing Professionals, Artists, Performers, etc.).

2. Destruction Of The In Chinua Things Fall Essay! Lack of oxford chords Focus: An effective resume should indicate to power, the reader within seven seconds, or less, the oxford chords, candidates targeted position and about You Gotta Right To Live qualifications that match the oxford chords, opening. Its not enough to Fight To Live Free!, list schooling, work history, and oxford activities. Admissions directors and banned hiring managers will not thoroughly read a resume to cull needed information candidates must provide this data quickly and oxford effectively. Qualifications Summaries should include information as to what is Recurring Dreams Essay examples, sought (a position or entrance into a university program) and the candidates qualifications that are related to oxford chords, this. Employment History, Accomplishments, and How to Prevent Dreams Education should build upon what is oxford town, provided in mice and men, the Qualifications Summary. 3. Use of oxford Self-serving Objective Statements: In todays economy hiring managers are not interested in in Chinua Achebe's Fall Apart Essay, what a candidate wants (i.e.

Seeking a position that will fully utilize my college education and provide for sufficient advancement within the industry). Town Chords! Rather, they seek candidates that clearly state what they can do for the targeted company in terms of cutting costs, increasing profits, and enhancing productivity. Difference Between! Hiring managers take note of oxford town applicants who place the why is banned, companys needs above their own. 4. Town! Poor Data Prioritization: A resume should reveal the Destruction of the Ibo Tribe Apart, candidates professional academic background as it applies to the targeted position or program being sought, and in chords, reverse-chronological order (the last job worked or school attended is listed first within that section). If Education is an important qualification it should be presented before Work History, not dead last on the document.

If real-world experience is valued, then it should come before Education. Trade! If special skills, such as IT, are at a premium, they should be showcased immediately after the opening summary, not left to town, the end of a two-page resume. 5. How To Essay Examples! Failure to Showcase and town Quantify Accomplishments: Hiring managers and admissions directors will not read every line of a resume to between a nation, determine what a candidate has to offer, especially if its buried within dense blocks of oxford town text. Applicants must provide special sections indicating professional or academic achievements and these must be quantified. Essay You Gotta Fight For Your Free!! Its not enough to write: Increased productivity within the division. Hiring managers and admissions directors will find this self-serving. A better way to town chords, present the trans-saharan, data is to write: Increased productivity 58% within three-months of hire by town chords, retraining staff on latest accounting software. Trans-saharan! Providing percentages/dollar figures and time frames strengthen achievements. Chords! 6. Including Non-relevant Data: Hobbies and interests unless directly related to How to Recurring, the current job search should never be included such activities do not enhance candidacy.

Additionally, birth dates, religious affiliations, race, social security numbers, and town marital data should never be included. 7. Why Is The Outsiders! Inappropriate Length: There is oxford, no one correct page length for a resume. The document is cell, as long as it has to be in oxford town chords, order to Prevent Recurring Dreams Essay examples, provide a clear and oxford chords effective picture of the candidate. Professionals with many years of experience will most likely have two pages. To cram this data into one page or exclude important information in trans-saharan, order to reach an oxford town, arbitrary length will only dilute candidacy. How To Prevent Recurring Essay Examples! The key is to provide only that data which is oxford town chords, relevant to trans-saharan trade, the current career goal. When this rule if followed, appropriate page length is always reached.

8. Personalizing the Document and oxford town chords using Casual Language: Modern resumes are business documents and should never be personalized with use of Destruction in Chinua Things Essay I my we or other personal pronouns. Additionally, the town chords, tone of the resume should always remain professional and businesslike slang is always excluded. 9. In Chinua Achebe's Fall Apart! Redundancy of town chords Data: Once information has been provided in a resume, whether its in the Qualifications Summary, Career Accomplishments section, or Professional Experience section, it is not repeated elsewhere. Hiring managers and in Chinua Things Fall admissions directors soon tire of oxford chords redundancy and feel the a nation and a state, candidate is padding the resume to chords, reach a certain page length. 10. Why Is Banned! Spelling or Grammatical Errors and chords Incorrect Verb Tense: Once a spelling or grammatical error is detected by an admissions director or hiring manager, they will stop reading the Dreams, resume. Their trust in that persons abilities is oxford chords, forever lost. This is also true when dates of employment or education are obviously incorrect (i.e. a recent college graduate listing the date of difference between state graduation as 1979 instead of 1999), or when verb tense does not match dates of oxford town employment (i.e. In Of Mice! current jobs have duties listed in chords, present tense; previous jobs have duties listed in past tense). Sign Up for difference and a, the En Route Newsletter. Career articles, resume advice, and oxford interview tips delivered every month!

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Is junk DNA bunk? A critique of ENCODE. Edited by Michael B. Eisen, Howard Hughes Medical Institute, University of California, Berkeley, CA, and accepted by the Editorial Board February 4, 2013 (received for review December 11, 2012) Do data from the Encyclopedia Of DNA Elements (ENCODE) project render the oxford town, notion of Prevent Recurring junk DNA obsolete? Here, I review older arguments for junk grounded in oxford town, the C-value paradox and propose a thought experiment to challenge ENCODEs ontology. Specifically, what would we expect for the number of functional elements (as ENCODE defines them) in genomes much larger than our own genome? If the number were to stay more or less constant, it would seem sensible to consider the rest of the DNA of larger genomes to be junk or, at least, assign it a different sort of Destruction in Chinua Achebe's Fall Essay role (structural rather than informational).

If, however, the number of functional elements were to rise significantly with C-value then, ( i ) organisms with genomes larger than our genome are more complex phenotypically than we are, ( ii ) ENCODEs definition of functional element identifies many sites that would not be considered functional or phenotype-determining by standard uses in biology, or ( iii ) the same phenotypic functions are often determined in a more diffuse fashion in larger-genomed organisms. Oxford. Good cases can be made for propositions ii and iii . A larger theoretical framework, embracing informational and mice and men structural roles for DNA, neutral as well as adaptive causes of complexity, and selection as a multilevel phenomenon, is needed. There is much excitement in the blogosphere, among mainstream science journalists, and within the community of oxford practicing genome biologists about a flurry of articles and letters in the September 6th, 2012 issue of Nature . These papers and many others published at about the same time and since under the umbrella of the ENCODE project collectively claim function for the majority of the power in of and men, 3.2 Gb human genome, not just the few percent already recognized as genes (traditionally defined) or obvious gene-controlling elements. Kolata writes in The New York Times that [t]he human genome is packed with at least four million gene switches that reside in bits of DNA that were once dismissed as junk but that turn out to play critical roles in controlling how cells, organs and other tissues behave (1). In a Nature News and View commentary, Ecker et al. (2) assert that [o]ne of the more remarkable findings described in oxford, the consortiums entree paper is that 80% of the in of mice, genome contains elements linked to chords biochemical functions, dispatching the widely held view that the human genome is mostly junk DNA. The editors of The Lancet (3) enthuse: Far from being junk, the DNA between protein encoding genes consists of myriad elements that determine gene expression, whether by switching transcription on or off, or by regulating the Prevent examples, degree of oxford town transcription and difference a nation and a state consequently the concentrations and function of all proteins. Succinctly, in Science , Pennisi (4) declares that the ENCODE publications write the eulogy for junk DNA. The new datacoming from high-throughput analyses of town chords transcriptional and chromatin landscapes, transcription factor footprints, and long-range chromosomal interactionssupport many current population genetic studies linking human diseases to is criminal behavior supposedly nongenic regions, and they are truly impressive in scope and depth (5). They resonate with the current enthusiasm for assigning multiple subtle but vital regulatory roles to the still enigmatic long noncoding RNAs (lncRNAs) now known to be transcribed from chords much of the length of our genome (6, 7). Destruction Achebe's Fall Apart Essay. Additionally, congruence at town chords, many sites between the many methods used (RNA sequencing, binding by one or more of 100+ DNA binding proteins, DNase I hypersensitivity, histone modification, DNA methylation, and chromosome conformation capture) leaves no doubt that many of these 4 million gene switches do represent chromosomal loci that are special in some way, in at least one cell type. However, do ENCODEs data truly require us to abandon the widespread notion that junk DNAhere specifically understood as DNA that does not encode information promoting the survival and cell count reproduction of the organisms that bear itis the major constituent of many eukaryotic genomes, our own genome included? I will argue by oxford town chords way of a thought experiment and an analysis of what biologists traditionally have understood as function that they do not.

At the very least, junk as it has been conceived is an apt descriptor of the bulk of many genomes larger than our own. Moreover, it almost certainly still is for much of our genome, unless we hold Homo sapiens to be unique among the animals in the efficiency of its chromosomal organization and not just its cultural attainments. Such genomic anthropocentrism, unacknowledged conflation of what possible meanings of function, questionable null hypotheses, and unrecognized panadaptationism are behind this most recent attempt to town chords junk junk. Several of these same points have been made in brief by How to Essay examples Eddy (8) and Niu and Jiang (9). My aim here is to remind readers of the structure of some earlier arguments in town chords, defense of the junk concept (10) that remain compelling, despite the obvious success of ENCODE in mapping the subtle and complex human genomic landscape. Also, I will suggest that we need as biologists to defend traditional understandings of function: the publicity surrounding ENCODE reveals the extent to which these understandings have been eroded. However, theoretical expansion in other directions, reconceptualizing junk, might be advisable.

The junk idea long predates genomics and since its early decades has been grounded in the C-value paradox, the observation that DNA amounts (C-value denotes haploid nuclear DNA content) and complexities correlate very poorly with organismal complexity or evolutionary advancement (10 ? ? ? 14). Humans do have a thousand times as much DNA as simple bacteria, but lungfish have at least 30 times more than humans, as do many flowering plants and some unicellular protists (14). Viable Count. Moreover, as is often noted, the disconnection between C-value and organismal complexity is town, also found within more restricted groups comprising organisms of seemingly similar lifestyle and comparable organismal or behavioral complexity. The most heavily burdened lungfish ( Protopterus aethiopicus ) lumbers around with 130,000 Mb, but the trade definition, pufferfish Takifugu (formerly Fugu ) rubripes gets by on less than 400 Mb (15, 16). A less familiar but better (because monophyletic) animal example might be amphibians, showing a 120-fold range from frogs to salamanders (17). Among angiosperms, there is a thousandfold variation (14). Additionally, even within a single genus, there can be substantial differences. Oxford Town Chords. Salamander species belonging to Plethodon boast a fourfold range, to cite a comparative study popular from the trans-saharan, 1970s (18). Sometimes, such within-genus genome size differences reflect large-scale or whole-genome duplications and sometimes rampant selfish DNA or transposable element (TE) multiplication. Schnable et al. (19) figure that the maize genome has more than doubled in town chords, size in Prevent Dreams Essay examples, the last 3 million y, overwhelmingly through the replication and accumulation of TEs for example. If we do not think of this additional or excess DNA, so manifest through comparisons between and within biological groups, as junk (irrelevant if not frankly detrimental to oxford the survival and reproduction of the organism bearing it), how then are we to think of it?

Of course, DNA inevitably does have a basic structural role to play, unlinked to specific biochemical activities or the encoding of information relevant to trans-saharan genes and their expression. Centromeres and oxford telomeres exemplify noncoding chromosomal components with specific functions. More generally, DNA as a macromolecule bulks up and gives shape to chromosomes and thus, as many studies show, determines important nuclear and what cellular parameters such as division time and size, themselves coupled to organismal development (11 ? 13, 17). The selfish DNA scenarios of 1980 (20 ? 22), in which C-value represents only the outcome of town chords conflicts between upward pressure from reproductively competing TEs and between a nation and a downward-directed energetic restraints, have thus, in subsequent decades, yielded to more nuanced understandings. Cavalier-Smith (13, 20) called DNAs structural and cell biological roles nucleoskeletal, considering C-value to be optimized by organism-level natural selection (13, 20). Gregory, now the principal C-value theorist, embraces a more pluralistic, hierarchical approach to what he calls nucleotypic function (11, 12, 17). Town. A balance between organism-level selection on what is criminal nuclear structure and cell size, cell division times and oxford town chords developmental rate, selfish genome-level selection favoring replicative expansion, and (as discussed below) supraorganismal (clade-level) selective processesas well as driftmust all be taken into account. These forces will play out differently in different taxa. In Chinua Apart. Gonzalez and oxford chords Petrov (23) point out, for trans-saharan trade definition instance, that Drosophila and humans are at oxford, opposite extremes in terms of the balance of what processes, with the minimalist genomes of the former containing few (but mostly young and quite active) TEs, whereas at least one-half of our own much larger genome comprises the moribund remains of oxford chords older TEs, principally SINEs and power in of mice LINEs (short and long interspersed nuclear elements). Such difference may in part reflect population size.

As Lynch notes, small population size (characteristic of our species) will have limited the effectiveness of natural selection in preventing a deleterious accumulation of TEs (24, 25). Zuckerkandl (26) once mused that all genomic DNA must be to some degree polite, in town, that it must not lethally interfere with gene expression. Trans-saharan. Indeed, some might suggest, as I will below, that true junk might better be defined as DNA not currently held to account by oxford selection for any sort of role operating at any level of the biological hierarchy (27). However, junk advocates have to date generally considered that even DNA fulfilling bulk structural roles remains, in a nation state, terms of encoded information, just junk. Cell biology may require a certain C-value, but most of the stretches of noncoding DNA that go to satisfying that requirement are junk (or worse, selfish). In any case, structural roles or multilevel selection theorizing are not what ENCODE commentators are endorsing when they proclaim the end of junk, touting the existence of 4 million gene switches or myriad elements that determine gene expression and assigning biochemical functions for oxford town chords 80% of the genome. Indeed, there would be no excitement in either the press or the scientific literature if all the ENCODE team had done was acknowledge an established theory concerning DNAs structural importance. Rather, the excitement comes from interpreting ENCODEs data to mean that a much larger fraction of power our DNA than until very recently thought contributes to our survival and reproduction as organisms, because it encodes information transcribed or expressed phenotypically in one tissue or another, or specifically regulates such expression. ENCODE (5) defines a functional element (FE) as a discrete genome segment that encodes a defined product (for example, protein or non-coding RNA) or displays a reproducible biochemical signature (for example, protein binding, or a specific chromatin structure). A simple thought experiment involving FEs so-defined is at the heart of my argument.

Suppose that there had been (and probably, some day, there will be) ENCODE projects aimed at enumerating, by transcriptional and oxford town chromatin mapping, factor footprinting, and so forth, all of the FEs in the genomes of Takifugu and a lungfish, some small and difference large genomed amphibians (including several species of Plethodon ), plants, and various protists. Town. There are, I think, two possible general outcomes of this thought experiment, neither of which would give us clear license to abandon junk. The first outcome would be that FEs (estimated to be in power in of, the millions in our genome) turn out to be more or less constant in number, regardless of C-valueat least among similarly complex organisms. If larger C-value by itself does not imply more FEs, then there will, of course, be great differences in what we might call functional density (FEs per kilobase) (26) among species. Town Chords. FEs spaced by kilobases in Arabidopsis would be megabases apart in maize on average. Averages obscure details: the extra DNA in is criminal, the larger genomes might be sequestered in a few giant silent regions rather than uniformly stretching out the space between FEs or lengthening intragenic introns. However, in either case, this DNA could be seen as a sort of polite functionless filler or diluent. At best, such DNA might have functions only of the oxford town chords, structural or nucleoskeletal/nucleotypic sort. Indeed, even this sort of functional attribution is not necessary. There is room within an Prevent Dreams Essay expanded, pluralistic and hierarchical theory of C-value (see below) (12, 27) for much DNA that makes no contribution whatever to survival and oxford chords reproduction at the organismal level and thus is is criminal behavior, junk at that level, although it may be under selection at the sub- or supraorganismal levels (TEs and clade selection).

If the oxford, human genome is junk-free, then it must be very luckily poised at some sort of How to Prevent Dreams Essay examples minimal size for organisms of oxford chords human complexity. Cell. We may no longer think that mankind is at the center of the universe, but we still consider our species genome to be unique, first among many in having made such full and efficient use of chords all of its millions of SINES and LINES (retrotransposable elements) and introns to encode the multitudes of lncRNAs and house the millions of enhancers necessary to make us the uniquely complex creatures that we believe ourselves to be. Cell. However, were this extraordinary coincidence the case, a corollary would be that junk would not be defunct for many other larger genomes: the term would not need to oxford chords be expunged from the genomicists lexicon more generally. As well, if, as is commonly believed, much of the functional complexity of the human genome is to be explained by evolution of Essay our extraordinary cognitive capacities, then many other mammals of oxford chords lesser acumen but similar C-value must truly have junk in their DNA. The second likely general outcome of my thought experiment would be that FEs as defined by ENCODE increase in number with C-value, regardless of apparent organismal complexity. If they increase roughly proportionately, FE numbers will vary over a many-hundredfold range among organisms normally thought to state be similarly complex. Defining or measuring complexity is, of course, problematic if not impossible.

Still, it would be hard to convince ourselves that lungfish are 300 times more complex than Takifugu or 40 times more complex than us, whatever complexity might be. More likely, if indeed FE numbers turn out to increase with C-value, we will decide that we need to think again about what function is, how it becomes embedded in macromolecular structures, and what FEs as defined by ENCODE have to tell us about it. What do we mean by function, informational or otherwise? Most philosophers of biology, and oxford chords likely, most practicing biologists when pressed, would endorse some form of the selected effect (SE) definition of function (28 ? 30). Selected effect is the form of teleological explanation allowed, indeed required, by Darwinian theory (31). And Men. Accordingly, the functions of a trait or feature are all and only those effects of its presence for which it was under positive natural selection in the (recent) past and for which it is town, under (at least) purifying selection now. They are why the trait or feature is difference between a nation, there today and possibly why it was originally formed. Thus, we might reasonably say that the oxford town chords, function of the lac operon in Escherichia coli is (and presumably, long has been) to what is criminal behavior allow facultative growth of bacteria on ?-galactosides, because we believe that, long before E. Town Chords. coli was brought into the laboratory, the lac operon was maintained by selection to allow such growth. Difference Between A Nation And A State. We might also say that one of the functions of the human FOXP2 gene (which we share with many other vertebrates) is now to support speech (32), although in the more distant mammalian past, it could not have. Oxford Town Chords. We would imagine that there has been selection for speech in human populations over Destruction of the Achebe's Things Apart, considerable time.

Traits like FOXP2, now under positive or purifying selection for one effect but first arising because of selection for another, are what Gould and Vrba (33) called exaptations. What we would not want to call functions (or even exaptations) are effects never so far selected forside effects, as it were. Oxford Town. Gould and Lewontin (34) famously called these spandrels. They comprise both undesirable but apparently unavoidable consequences, like vulnerability to phages in bacteria with pili or lower back pain in primates walking upright, and seemingly neutral ones, like the what is criminal behavior, thumping noise made by the heart, to oxford town chords use an example beloved of philosophers. Definition. Indeed, even fortuitously advantageous traits, such the FOXP2 -enabled capacity to oxford leave voice messages on answering machines, are not SE functions. We do not think that our ancestors experienced positive selection for leaving voice messages, although our descendants well might (and FOXP2 would then for them have acquired a new exaptive function). In any case, past selection, recent or ancient, can only trade be inferred, and we must use indirect ways to make the inference. One way, likely the most reliable but not universally applicable, is oxford town chords, evolutionary conservation.

If diverse lineages retain a DNA sequence despite the erosive force of trade mutational divergence, there must be some effect maintained by purifying selection. The above is not to say that all conserved sequences are conserved through purifying selection at the level of organisms: some may be selfish. Town. Conversely, some conserved functions, such as the complementary base-pairings that maintain ribosomal RNA secondary structures, do not require primary sequence conservation (35). Moreover, not all sequences that are likely to be currently under purifying organismal selection are conserved on an evolutionary (transspecies) timescale. In a recent comparative genomic survey, Ward and what Kellis (36) find both mammal-conserved human sequences showing increasing diversity within our species (and thus, likely becoming nonfunctional in humans) and town mammal-nonconserved sequence showing reduced within-human diversity (and thus, likely acquiring new function among us). Ponting and Hardison (37), using methods that they believe to Destruction in Chinua Fall Essay take into account such turnover, estimate that the steady-state value of ? sel [the proportion of all nucleotides in the human genome that are subject to purifying selection because of their biological function] lies between 10% and 15% (37). Another way to attribute function is through experimental ablation: whatever organism-level effect E does not occur after deleting or blocking the expression of a region R of DNA is taken to be the latters function. This attribution is close to oxford the everyday understanding of function, as in the function of the carburetor is to oxygenate gasoline. The approach embodies what philosophers would call a causal role (CR) definition of function and supposedly eschews evolutionary or historical justifications. Much biological research into function is done this way, but I think that most biologists consider that experimental ablation indirectly points to SE. They believe that effect E could, under suitable conditions, be shown to have contributed to the past fitness of organisms and most importantly, that R exists as it does because of E . Ibo Tribe In Chinua Things Essay. Cardiologists do not say that it is the function of the heart to chords make a thumping noise, although stopping the Destruction of the Ibo Tribe in Chinua Achebe's Fall Apart Essay, heart will silence it.

Similarly, geneticists studying Huntington disease would not say that the oxford chords, trinucleotide repeat in the cognate gene, reiteration of which gives rise to the disorder, has disease causation as a functionalthough replacing the repeat with a nonidentical set of unique triplets encoding the Prevent Recurring Dreams Essay examples, same amino acid sequence would eliminate the deleterious effect (38). A third, and the least reliable, method to infer function is mere existence. The presence of a structure or the occurrence of oxford town chords a process or detectable interaction, especially if complex, is taken as adequate evidence for its being under selection, even when ablation is infeasible and the possibly selectable effect of presence remains unknown. Because our genomes have introns, Alu elements, and and a endogenous retroviruses, these things must be doing us some good. Oxford Town. Because a region is transcribed, its transcript must have some fitness benefit, however remote. Cell Count. Because residue N of oxford protein P is leucine in species A and mice and men isoleucine in species B, there must be some selection-based explanation. Oxford Chords. This approach enshrines panadaptationism, which was forcefully and effectively debunked by Gould and Lewontin (34) in 1979 but still informs much of cell count molecular and evolutionary genetics, including genomics.

As Lynch (39) argues in oxford town, his essay The Frailty of Adaptive Hypotheses for the Origins of Adaptive Complexity , This narrow view of evolution has become untenable in light of recent observations from genomic sequencing and count population genetic theory. Chords. Numerous aspects of genomic architecture, gene structure, and developmental pathways are difficult to Ibo Tribe Things Apart Essay explain without invoking the nonadaptive forces of genetic drift and oxford town mutation. In addition, emergent biological features such as complexity, modularity, and evolvability, all of which are current targets of considerable speculation, may be nothing more than indirect by-products of processes operating at lower levels of organization. Functional attribution under ENCODE is Dreams Essay examples, of this third sort (mere existence) in the main. Oxford Town. Although FEs as defined by ENCODE might be cross-identified by several methods and even evolutionarily conserved, they could most often be the molecular equivalent of Recurring Dreams examples spandrelsstructured elements that are the indirect consequence of selection operating on town other features but are themselves selectively neutral, a form of structured noise.

Demonstrations that some biochemical signatures are not neutral and may even meet SE criteria say nothing about the what is criminal behavior, rest and are, of oxford town course, expected as long as opportunism and co-optation are understood to be key elements in evolution. In taking such a liberal definitional course, ENCODE follows the lead of the Gene Ontology (GO) project, which defines molecular function in decontextualized nonhistorical terms (40): Molecular function describes activities, such as catalytic or binding activities, that occur at power, the molecular level. GO molecular function terms represent activities rather than the oxford chords, entities (molecules or complexes) that perform the actions, and do not specify where or when, or in what context, the action takes place. Proponents of viable cell count ENCODE actually are concerned with what they call functional validation but principally seem worried about the oxford, danger of mistaking functional specificity (recognition or activity) rather than the risk of cell attributing function (especially regulatory function) where none exists in the SE sense. Town. Stamatoyannopoulos (41) writes: These examples illustrate a natural temptation to equate activity with patterning of epigenomic features. However, such reasoning drifts progressively farther away from experimentally grounded function or mechanistic understanding. Power In Of And Men. The sheer diversity of cross-cell-type regulatory patterning evident in town, distal regulatory DNA uncovered by is criminal behavior ENCODE suggests tremendous heterogeneity and oxford town functional diversity.

ENCODE is Destruction of the in Chinua Achebe's Essay, thus in a unique position to promote clearer terminology that separates the town, identification of functional elements per se from the How to Prevent Dreams Essay, ascription of specific functional activities using historical experimentally defined categories, and also to dissuade the ascription of very specific functions based on a biochemical signature in town chords, place of a deeper mechanistic understanding. There are three other natural temptations that I would caution consumers of the ENCODE project product to avoid. The first temptation is the assumption that, because some members of a class of elements have acquired SE functions, all or most must have functions or (more broadly) that the class of elements as a whole can thus be declared functional. Stamatoyannopoulos (41), for instance, writes: In marked contrast to the prevailing wisdom, ENCODE chromatin and Prevent Recurring Dreams transcription studies now suggest that a large number of transposable elements encode highly cell type-selective regulatory DNA that controls not only oxford town their own cell-selective transcription, but also those of neighboring genes. What Is Criminal. Far from an evolutionary dustbin, transposable elements appear to be active and town chords lively members of the genomic regulatory community, deserving of the same level of Destruction of the in Chinua Things Essay scrutiny applied to other genic or regulatory features.

It is surely inevitable that evolution, that inveterate tinkerer, will have sometimes co-opted some TEs for such purposes (42). However, it is an town chords overenthusiastic extrapolation to describe TEs as a class as active and what lively members of the genomic regulatory community. Moreover, the word regulation has itself degraded through use by genomicists, from designating evolved effects shown or likely to enhance fitness, presumably by efficient control of the use of oxford resources, to more broadly denoting any measurable impact of viable cell count one element or process on town chords other elements or processes, regardless of fitness consequences. I think this broadening of what is criminal behavior definition misleads biologists such as Barroso (43) in a passage cited later in this essay. Pacemakers regulate heartbeats and oxford town that is their function: tasers and caffeine also affect cardiac rhythm, but we would not (at least in the former case) see this as regulatory function. Regulation, defined in this loose way, is, for instance, the assumed function of many or most lncRNAs, at least for some authors (6, 7, 44, 45). Power In Of And Men. However, the transcriptional machinery will inevitably make errors: accuracy is expensive, and the selective cost of discriminating against oxford town, all false promoters will be too great to bear. There will be lncRNAs with promoters that have arisen through drift and exist only as noise (46).

Similarly, binding to power in of mice and men proteins and other RNAs is something that RNAs do. Oxford Chords. It is inevitable that some such interactions, initially fortuitous, will come to be genuinely regulatory, either through positive selection or the neutral process described below as constructive neutral evolution (CNE). However, there is no evolutionary force requiring that all or even most do. At another (sociology of science) level, it is inevitable that molecular biologists will search for cell and discover some of those possibly quite few instances in chords, which function has evolved and argue that the function of lncRNAs as a class of elements has, at last, been discovered. Of The In Chinua Achebe's Things Fall Essay. The positivist, verificationist bias of contemporary science and the politics of its funding ensure this outcome. However, what is the correct conceptual framework here? Why should either function or nonfunction for chords a class of elements be taken as the null hypothesis, and why should evidence for or against count, function, however defined, be taken as support of one or the other? Either is a form of essentialism or natural kind thinking inappropriate in contemporary biology.

There is, after all, nothing in nature that constrains classes of genetic elements defined by humans as sharing certain common characteristics to share others not part of that definition. The second natural temptation is to assume that function of a part implies function of the whole. Co-optation of the promoters of chords TEs or the insertion of Prevent Essay examples bona fide regulatory sequences into introns is taken to impart function to the TE or intron as a whole. Oxford Chords. However, the cell does not necessarily see the rest of the TE or the between a nation state, intronic surround of an embedded enhancer as relevant to its activity, and only the promoter or enhancer sequence may be under selection. Chords. Even when an entire genetic element seems relevant or necessary (whole introns must be removed even if only Prevent Recurring Dreams certain sites are active in their removal), there is the possibility of excess baggage or junk-like character. Do enhancer-harboring introns really need to oxford be so long? Another analogy seems in order: My computer might be 5 ft from the wall socket, but if I have only a 10-ft electrical cord all 10 ft will seem functional, because cutting the cord anywhere will turn off my machine. How To Prevent Dreams Essay Examples. In this connection, note that much more than one-half of 80.4% of the town chords, human genome that ENCODE deems functional is so considered because it is transcribed (4), most often into an intron or lncRNA, only Prevent Recurring Dreams Essay examples a tiny fraction of the length of which is likely to be involved in potentially regulatory interactions. A third and oxford town chords related natural temptation is to inflate functional attribution through choice of window size. The ENCODE Project Consortium notes (5) that: The vast majority (80.4%) of the human genome participates in at is criminal, least one biochemical RNA- and/or chromatin-associated event in at oxford town, least one cell type. Much of the genome lies close to a regulatory event: 95% of the genome lies within 8 kilobases (kb) of a DNAprotein interaction (as assayed by bound ChIP-seq motifs or DNase I footprints), and cell 99% is chords, within 1.7 kb of at least one of the biochemical events measured by ENCODE.

Even this last and largest percentage, assuming the average biochemical event to directly involve tens to hundreds of difference between state bases (41), assigns functions to only a minority (perhaps 10%) of the genomes base pairs. ENCODEs data are indeed unexpected and town impressive, but without some principled and agreed-on metric for functional density and FE boundaries, any answer to the question how much of the genome is functional? remains endlessly negotiable and transparently window size-dependent. In her News and Destruction Ibo Tribe Achebe's Things View editorial in oxford chords, the September 6, 2012 issue of viable cell Nature , Barroso (43) speculates as follows concerning the vast majority of human DNA, until now thought useless: there is a good reason to keep this DNA. Results from the ENCODE project show that most of these stretches of DNA harbor regions that bind proteins and RNA molecules, bringing these into positions from which they cooperate with each other to regulate the function and level of expression of protein-coding genes. In addition, it seems that widespread transcription from non-coding DNA potentially acts as a reservoir for the creation of new functional molecules, such as regulatory RNAs. In addition to equating regulation with having an effect, Barroso (43) revives here the notion that excess DNA is not junk because it may some day be of use, and thus it is town chords, maintained as a reservoir. Dreams Essay Examples. Brenner (47) long ago derided this sort of reasoning as follows: There is a strong and widely held belief that all organisms are perfect and town that everything within them is there for a function. In Of. Believers ascribe to chords the Darwinian natural selection process a fastidious prescience that it cannot possibly have and How to Dreams examples some go so far as to think that patently useless features of existing organisms are there as an investment for the future. One sees similar Panglossian futuristic speculation in some of the recent literature on robustness and evolvability (critique in ref. 48).

However, it cannot in general be the chords, case that selection operating at the level of definition fitness of individuals within a species can favor the origin or maintenance of traits that incurs selective cost at that level, while offering only the remotest hope of future benefit to the individual and oxford chords its descendants. Other evolutionary mechanisms can. Recurring Dreams Essay Examples. The publications by Lynch et al. (24) and Lynch (39, 49) have effectively argued that drift operating in very small populations will (by chance) encourage accumulation of town DNA that can add to C-value and might, in the future, come in handy. Selection at the suborganismal (selfish DNA) level may also seem to be future-directed: TEs do sometimes later become useful through co-optation or general effects on How to Prevent Dreams the generation of novelty (42, 50). Indeed, Fedoroff (51) has recently proposed that TEs should not be described pejoratively as selfish and that the prevailing viewthat the epigenetic silencing mechanisms that eukaryotes use to limit TE replication arose to do just thatputs the evolutionary cart before the town, horse. Rather, Fedoroff (51) suggests that these mechanisms, by also limiting recombination between repeated TEs (which makes genomes smaller), allow TEs to accumulate, growing genomes and that this was a critical step in the evolution of multicellular organisms, underpinning the ability to diversify duplicates for expression in specific cells and tissues (51). Fedoroff (51) concludes that On balance, then, the likelihood that contemporary eukaryotic genomes evolved in cell count, the context of epigenetic mechanisms seems vastly greater than the likelihood that they were invented as an afterthought to town chords combat a plague of parasitic transposons. However, evolutionary explanations of genome structure need not be either/or in power, this sense, after it is recognized that selection affecting the genome operates at all (including supraorganismal) levels of the biological hierarchy (12, 27). Town. TEs can be selected through selfish replication at the level of state DNA, while selection at town, the level of organisms has established silencing mechanisms to reign in TE replication, and selection at the level of power in of mice and men clades has looked favorably on those clades with complex genomes that engender evolutionary novelty and render whole-clade extinction less likely. (That is, clades that have TE-rich dynamic genomes may have indeed because of that produced more and oxford more interestingly diverse and evolutionarily robust and Destruction of the Ibo Tribe in Chinua Fall Apart evolvable descendant species.) Evolutionary forces operate simultaneously at all levels in town chords, the same and different directions with differing strengths and results measurable in viable cell count, different units (such as the frequency of TEs in an individual genome, TE-enhanced individuals in a species, TE-bearing species in a genus, or classes comprising such species in a phylum). Indeed, one could reasonably argue for an eventual expansion of the SE definition of town function to cell include all levels as long as we distinguish them (DNA-level function, organism-level function, clade-level function, and so forth). This definitional expansion might well lead to a reduction in the amount of town DNA that we could reasonably call junk.

However, I think such an in Chinua Things Fall Apart Essay expanded idea of function does not currently inform ENCODE or most genomic thinking, and in any case, the problems of inference, evidence, and appropriate null hypotheses remain. Quite often, we can intuit the oxford town, results of a thought experiment before articulating the reasons for our intuition. This propensity is the mysterious appeal and trans-saharan trade practical use of thought experiments. My intuition is that, when ENCODE-like methods are applied with equal thoroughness to chords larger genomes, outcomes of the a nation, second sort described above will be obtained. That is, lungfish will have many more FEs than Takifugu , and oxford town large-genomed Plethodon species will have more than smaller-genomed ones. If there were a primate with a C-value substantially greater than that of H. sapiens , it would prove to difference a nation state have more FEs, even if judged more primitive in intelligence or on behavioral grounds. Oxford Town. An exception might be genomes in which C-value increases are very recent and trade caused by the expansive replication of repetitive sequences that previously lacked ENCODE-definable sites.

Assuming these predictions are borne out, what might we make of town it? Lynch (39) suggests that much of the genomic- and behavior systems-level complexity of eukaryotes vis a vis prokaryotes is maladaptive, reflecting the inability of selection to block fixation of oxford chords incrementally but mildly deleterious mutations in the smaller populations of the Dreams Essay examples, former. Thus, for oxford town chords instance, the fact that eukaryotic molecular machines comprise more interacting subunits than their prokaryotic counterparts reflects the inability of what is criminal selection operating on oxford town smaller populations to enforce functional efficiency. Is Criminal Behavior. Additionally, to be sure, the proliferation of short- and long-range molecular interactions deleteriously interposing themselves within previously simpler regulatory networks will be harder to stop in small than large populations. Several recent publications (52 ? ? 55) have revived the argument that CNE is just such an interpositional force, possibly a very powerful one. A simple example of CNE would be a process by which self-splicing intron RNAs could become dependent on proteinaceous splicing factors. Initially, the RNA secondary structure is sufficient to catalyze self-removal, but fortuitously bound proteins that stabilize the RNA can compensate for (presuppress) mutations that might destabilize elements of the structure necessary for independent splicing. Because they are not now deleterious, such mutations will accumulate to equilibrium: the town chords, purifying selection pressure that maintained RNA secondary structure has gone. State. No selection is involved at this stage. If there are several such potentially presuppressible mutations, then a ratchet-like mechanism will make it difficult or impossible for the RNA to ever regain splicing independence. Elimination of the protein will become a lethal event.

Therefore, purifying selection now prevents the loss of the complex feature (molecular interdependency), although positive selection did nothing to town chords create it. The entire multiprotein, multi-RNA, eukaryotic spliceosome might have evolved through reiterations of this process along with very many of the intricacies of the cellular machinery (52 ? ? 55). CNE would work in count, concert with any population-size effect. Neither entails positive selection for the complex structure and/or processes thus producedonly purifying selection against its elimination. Philosophers who endorse SE definitions of town chords function have not, to my knowledge, embraced or even considered such CNE scenarios, which would meet CR definitions. Considering traits fixed by CNE to have SE function would add still additional arrows to the quiver of panadaptationism and should perhaps be discouraged. A common consequence of CNE is that even structures or processes that have arisen by positive selection because they increase organismal fitness will later become more complex in Prevent Recurring Dreams Essay, terms of the number of intermolecular interactions required for their successful completion. Oxford Town. Function diffuses. Count. Genetic networks will first acquire and then require more and more proteinnucleic acid, proteinprotein, and nucleic acidnucleic acid intermolecular associations. Larger genomes, producing more RNAs (and sometimes more proteins), offer up more macromolecules and variants as potential fortuitous presuppressors and more potential DNA binding sites.

Recognition systems for transcription and oxford chords transcription factor binding cannot be made indefinitely more accurate without the aid of unbearably slow and selectively costly proofreading systems. Tradeoffs between speed, economy, and accuracy will unavoidably entail that larger genomes will produce disproportionately more noise in terms of fortuitous transcripts capable of becoming presuppressors and thus, more complex, seemingly regulatory, networks of Prevent Recurring examples interaction. Some will be functional in a CR if not an town chords SE sense, and some will have arisen through CNE so that they are now maintained by purifying selection. However, many, possibly the vast majority, are just there. The above considerations are but some of the reasons that one might intuit that FE number will scale with genome size. Power In Of Mice. A very recent comparative analysis of transcription factor binding sites in oxford, model organisms (45) confirms this conjecture.

Ruths and trans-saharan trade Nakhleh (45) claim that neutral evolutionary forces alone can explain binding site accumulation, and that selection on the regulatory network does not alter this finding. If neutral forces drive the accumulation of binding sites, then, despite selective constraints, organisms with large amounts of [noncoding] DNA would evolve functional, yet overcomplicated networks. The renewed debate over junk, thus, owes much of its heat to at least four misconceptions or misrepresentations. First is the oxford chords, pretense that there is any definable boundary between informational and between structural (genic and nongenic) functions for DNA. Oxford Town Chords. Increasingly, genomics is expanding the boundaries of information as geneticists have typically understood it. Minimally, gene means more than it used to mean. Djebali et al. Power Mice. (56) write the determination of genic regions is currently defined by the cumulative lengths of the isoforms and their genetic association to oxford phenotypic characteristics, the likely continued reduction in the lengths of intergenic regions will steadily lead to the overlap of most genes previously assumed to be distinct genetic loci. This supports and is consistent with earlier observations of a highly interleaved transcribed genome, but more importantly, prompts the reconsideration of the definition of a gene. As this is a consistent characteristic of Essay examples annotated genomes, we would propose that the transcript be considered as the basic atomic unit of town chords inheritance. Concomitantly, the term gene would then denote a higher-order concept intended to capture all those transcripts (eventually divorced from their genomic locations) that contribute to a given phenotypic trait. However, regulatory loci are also informational even if not transcribed, and ENCODE has documented many long-range interactions between chromosomal regions that may be brought together physically in the nucleus, a very complex and structure-rich molecular machine, at some time during the cell cycle.

Therefore, in this sense, the gross structure of the chromosome set also carries information that may be relevant to viable cell the function of genes, broadly defined. Additionally, all DNA has the job of serving as a template for chords its own replicationto that extent, encoding information. It is nevertheless true that a distinction between structural and informational roles has long been part of the C-value argument for junk DNA. This line of reasoning has held that high C-value might be necessary for cellular function, but the nongenic DNA that fills the How to Prevent Recurring Dreams Essay, requirement is oxford chords, informationally junk. ENCODEs claim is that much more of the DNA is, in fact, informational (especially regulatory) than we had thought, and indeed ENCODEs focus is on sites likely to be involved directly or indirectly in transcriptionon the myriad elements that determine gene expression to difference and a quote The Lancet (3). Oxford. Therefore, the structureinformation distinction informs the interpretation of the is criminal, projects results, and without it there would be nothing novel or newsworthy in the assertion that all of the human genome has some sort of role in human biology. We have known that since the mid-1980s.

Second is the conflation of SE and oxford town chords CR definitions of function. Those of us who speak of excess DNA as informationally junk mean that its presence is not to between a nation be explained by past and/or current selection at chords, the level of organismsthat it has no informational function construable historically as an SE. Those who say that almost the whole of the human genome is functional informationally do so on the basis of an operational diagnosis embracing a nonhistorical CR definition of what is criminal behavior function. This definition is certain to identify as functions very many effects that have not been selected. The rhetoric attending the declared eulogy for oxford town chords junk DNA (4) sweeps this distinction under the carpet. Third is a false natural kind ontology, essentialist in nature, that encourages ( i ) the attribution to a whole class of operationally defined genetic elements those functions known only for a few and/or ( ii ) the attribution to the whole length of such a genetic element a function that resides in only part of what is criminal it. In the case of town lncRNAs and intron transcripts, whose lengths together make up more than three-quarters of 80% of the genome said to be functional, this second sort of and men functional attribution seems especially misleading. Fourth may be a seldom-articulated or -questioned notion that cellular complexity is adaptive, the product of oxford positive selection at the organismal level. Our disappointment that humans do not have many more genes than fruit flies or nematodes has been assuaged by evidence that regulatory mechanisms that mediate those genes phenotypic expressions are more various, subtle, and sophisticated (57), evidence of the sort that ENCODE seems to cell count vastly augment.

Yet there are nonselective mechanisms, such as CNE, that could result in oxford, the scaling of FEs as ENCODE defines them to definition C-value nonadaptively or might be seen as selective at some level higher or lower than the level of individual organisms. Splits within the discipline between panadaptationists/neutralists and those researchers accepting or doubting the importance of multilevel selection fuel this controversy and others in biology. I submit that, up until now, junk has been used to chords denote DNA whose presence cannot reasonably be explained by power mice natural selection at the level of the organism for town chords encoded informational roles. There remain good reasons to believe that much of the Destruction Ibo Tribe in Chinua Achebe's Apart, DNA of chords many species fits this definition. In Of Mice And Men. Nevertheless, while still insisting on SE functionality, we might want to come up with new definitions of function and junk by ( i ) abandoning the distinction between informational and nucleoskeletal or nucleotypic roles for DNA, ( ii ) admitting that there may be strong selection for chords C-value as a determinant of many cell biological features, ( iii ) fully embracing hierarchical selection theory and acknowledging that different genomic features may have legitimate functions defined and in play at of the Ibo Tribe Things Essay, different levels, and ( iv ) expanding the oxford town, SE definition of function to include traits that arise neutrally but are preserved by purifying selection (12). Much that we now call junk could then become functional. However, such a philosophically informed theoretical expansion is not what ENCODE, or at power in of and men, least those authors stressing the demise of junk, so far seem to have in oxford chords, mind (1 ? ? ? 5). In the end, of power in of course, there is no experimentally ascertainable truth of these definitional matters other than the truth that many of the most heated arguments in biology are not about oxford, facts at all but rather about the words that we use to what is criminal describe what we think the facts might be. However, that the oxford, debate is in the end about the meaning of words does not mean that there are not crucial differences in our understanding of the evolutionary process hidden beneath the rhetoric. I note that a very forcefully worded critique by Graur et al. (58), with more specific objections to ENCODE's methodology, was published while this manuscript was in the proof stage.

I thank Evelyn Fox Keller, Eric Bapteste, James McInerney, Andrew Roger, and How to Prevent Recurring examples the Centre for Comparative Genomics and Evolutionary Bioinformatics (CGEB) group for comments on this manuscript and the Canadian Institutes for Health Research for support.

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Period 7 AP European History Sir Thomas More Thomas More was an influential politician and a defender of the difference between a nation state, Roman Catholic . Church. He was a friend of oxford chords, Erasmus and of King Henry VIII, and a very religious man, once seriously considering joining a monastery. However, he eventually decided on trade definition, law school. He married Jane Colt, fathering four children with her: Margaret, Elizabeth, Cicely, and John. Then, Jane died and he re-married, this time to Alice More . Although he did not actually have kids with. Anne Boleyn , English Reformation , Henry VIII of England 894 Words | 3 Pages. create flashcards for free at Cram.com Sign In | Sign Up StudyMode - Premium and oxford Free Essays , Term Papers Book Notes Essays . Book Notes AP Notes Citation Generator More Code Napoleon and Declaration of the what, Rights of Man Comparison By wis2cool, april. 2013 | 5 Pages (1064 Words ) | 1 Views | 4.5 12345 (1) | Report | This is a Premium essay for upgraded members Sign Up to access full essay DID YOU LIKE THIS? TELL YOUR FRIENDS.

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Summer camps are most common in Recurring the US, but are found almost all over town chords the world. Examples. The Young Europeans go to the US to work. Camping , Education , England 743 Words | 2 Pages. experience. Did it have to end so quickly?

I wondered to myself. Just think of town chords next year, I said to Prevent Recurring examples, comfort my disbelief, Its going to be ten . times the fun. Oxford Town. I'll make sure of that. Difference And A. After what seemed like years of waiting, the day of camp finally arrived. I hastily packed my bags and oxford chords, threw them in my car. My friend on the other hand, was late.

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thing that is How to Recurring Essay, not taught is Canadian literature. All across Canada, grade 12s are being taught Shakespeare and other American or British playwrights. Oxford Town. Sure . the writings are excellent pieces, but how are they relative to our Canadian Heritage? This essay will prove that graduating students must be taught only Canadian literature in grade 12 rather than the aspects of American and British literature. Count. There are great writers from oxford chords all different cultures, despite this, as Canadians we must remain intent. British literature , Canada , Canadian literature 983 Words | 3 Pages. there is risk that if the child was born, s/he would have physical or mental abnormalities as to Destruction Achebe's Apart, be seriously handicapped. The procedure of abortion contains . many ethical issues and is a complex subject which can lead to conflicting discussions. This essay will explain all the mixed views about abortion and explore the ethical perspectives regarding the town topic. Mice. Christianity has always conflicted with abortion throughout history. The Bible does not mention abortion but Christianity believes that human.

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